A validated spectrophotometric method for the analysis of total flavonoids in propolis samples
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Abstract
Flavonoids are natural phytochemicals secondary products of plant’s metabolism. This analytes are part of propolis composition, bee product that honeybees made from exudates or plant’s resin as a result of beekeeping work. The antioxidant activity in propolis is related to its concentration of flavonoids. The determination of this substances require sensible and selective methods, further- more, the quality control and food safety regulations require the validation of these methods. Validation’s procedure supports the veracity of the results obtained in the laboratory analysis. Linearity, quantification limit, precision (repeatability and reproducibility), and accuracy, were the established validation parameters. The study of total flavonoids content was carried out through a colorimetric method with aluminum chloride as chelator agent. The color-complex formation allows the spectrophotometric measurement at 415 nm. This procedure was lineal within the range of 2.0-10.0 µg/mL with R2=0.9999. A total variation coefficient of 3.82, 2.28 and 3.61 % for propolis type I, II and III respectively was obtained during intermediate precision assay. Reproducibility showed a variation coefficient of 3.72 (type I), 3.00 (type II), and 2.66 % (type III). The recovery was in the range of 79.1-101.9 % (considering the three propolis type). Finally, the quantification limit obtained for this procedure was 0.091 µg/mL.
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